|
週次 |
日期 |
單元主題 |
|
第1週 |
02/19 |
Introduction of Ubiquitin, PTM and other UBLs, protein homeostasis, role of cysteine in chemical reactions |
|
第2週 |
02/26 |
The E1 activation enzyme:
• Structural elements
• Chemical reaction, mechanism, and function |
|
第3週 |
03/04 |
Round table paper discussions (1) |
|
第4週 |
03/11 |
"The E2 conjugase:
• core structure and function
• canonical/non-canonical E2 conjugase
• role of backside binding
• N-terminal acetylation and activity" |
|
第5週 |
03/18 |
The E3 ligase (1): RING E3: function, structure, mechanism and case studies |
|
第6週 |
03/25 |
The E3 ligase (2): HECT E3: function, structure, mechanism and case studies |
|
第7週 |
04/01 |
The E3 ligase (3): RBR E3 (vs HECT, vs RING, the ubiquitin relay): function, structure and case studies |
|
第8週 |
04/08 |
"Ubiquitin chain (types, genration, and detection)
Deubiquitinase (DUB: function, structure, and mechansim)" |
|
第9週 |
04/15 |
"Deubiqutinase (continued)
Proteasome structure and function" |
|
第10週 |
04/22 |
"Other ubiquitin-like proteins (UBL)
• SUMO
• NEDD8 and crosstalks to Culin RING E3 ligase
• ISG15 and other UBLs
• Autophagy
• Viral/pathogenic ubiquitin-like system " |
|
第11週 |
04/29 |
"Ubiquitin probes and engineering
• Chemical probes for ubiquitin
• Ubiquitin variants
• advanced tools
• Ubiquitin and protein condensate (LLPS)" |
|
第12週 |
05/06 |
"Ubiquitin, diseases and drug discovery:
• PROTAC and protein degradation
• Inhibitor Drugs for E1, or E2
• Cognate-selected ubiquitin variants
• Selectivity and specificity of small molecular inhibitors" |
|
第13週 |
05/13 |
Round table paper discussions (2) |
|
第14週 |
05/20 |
Final Exam: Case representation (1) |
|
第15週 |
05/27 |
Final Exam: Case representation (2) |
|
第16週 |
06/03 |
Final Exam: Case representation (3) |